Pleurotus citrinopileatus خالص سازی و شناسایی یک گلوکان  قارچ صدفی طلایی

Purification and identification of one glucan from golden oystermushroom (Pleurotus citrinopileatus (Fr.) Singer)

Abstract

One water-soluble glucan (PCP-W1) was purified from the crude polysaccharide of Pleurotus citrinopileatus by chromatography on DEAE Sepharose Fast Flow and Sephadex G-200 column, and PCP-W1 (Mw = 45 kDa), was predominantly composed of Glc. Partial acid hydrolysis, Smith degradation–periodate oxidation, methylation analysis, Fourier transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC–MS) analysis and nuclear magnetic resonance spectroscopy (NMR) experiments were conducted to elucidate its structure. The results indicated that PCP-W1 had a glucan backbone consisting of (1 → 6)-linked-β-d-glucopyranosyl residues, which were branched at O-3 position of the backbone with (1 → 3)-linked-β-d-glucopyranosyl and non-reducing end 1-β-d-glucopyranosyl residues. The repeating unit of the polysaccharide was established as:

Highlights

► One glycan (PCP-W1) was purified from the fruiting bodies of Pleurotus citrinopileatus. ► The structural features were identified by the combination of chemical and instrumental analysis. ► PCP-W1 had a glucan backbone.