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ویتامین D4 در قارچ ها
Vitamin D4 in Mushrooms
Katherine M. Phillips 1*, Ronald L. Horst2, Nicholas J. Koszewski3, Ryan R. Simon4
Abstract
An unknown vitamin D compound was observed in the HPLC-UV chromatogram of edible mushrooms in the course of
analyzing vitamin D 2 as part of a food composition study and confirmed by liquid chromatography-mass spectrometry to be
vitamin D 4 (22-dihydroergocalciferol). Vitamin D4 was quantified by HPLC with UV detection, with vitamin [3H] itamin D3 as
an internal standard. White button, crimini, portabella, enoki, shiitake, maitake, oyster, morel, chanterelle, and UV-treated
portabella mushrooms were analyzed, as four composites each of a total of 71 samples from U.S. retail suppliers and
producers. Vitamin D 4 was present (.0.1 mg/100 g) in a total of 18 composites and in at least one composite of each
mushroom type except white button. The level was highest in samples with known UV exposure: vitamin D enhanced
portabella, and maitake mushrooms from one supplier (0.2–7.0 and 22.5–35.4 mg/100 g, respectively). Other mushrooms
had detectable vitamin D 4 in some but not all samples. In one composite of oyster mushrooms the vitamin D4 content was
more than twice that of D 2 (6.29 vs. 2.59 mg/100 g). Vitamin D4 exceeded 2 mg/100 g in the morel and chanterelle
mushroom samples that contained D 4, but was undetectable in two morel samples. The vitamin D4 precursor 22,23-
dihydroergosterol was found in all composites (4.49–16.5 mg/100 g). Vitamin D 4 should be expected to occur in
mushrooms exposed to UV light, such as commercially produced vitamin D enhanced products, wild grown mushrooms or
other mushrooms receiving incidental exposure. Because vitamin D 4 coeluted with D3 in the routine HPLC analysis of
vitamin D 2 and an alternate mobile phase was necessary for resolution, researchers analyzing vitamin D2 in mushrooms and
using D 3 as an internal standard should verify that the system will resolve vitamins D3 and D4.
:: موضوعات مرتبط:
مقالات آموزشی قارچ
:: برچسبها:
مقاله 2012,
ویتامین D4 در قارچ ها
ن : دکتر ولی اله مهدیزاده
ت : جمعه هجدهم فروردین ۱۳۹۱
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