Archives Of Phytopathology And Plant Protection

Available online: 30 Jan 2012

Intraspecies Diversity of Macrophomina phaseolina in Iran

V. Mahdizadeh¹, N. Safaie¹, E. Mohammadi Goltapeh¹ and N. Mayek-Perez ²

¹Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

²Centro de Biotecnología Genómica-Instituto Politécnico Nacional, Reynosa, Tamaulipas, México.

Abstract

            To study the pathogenic and genetic diversity of the Macrophomina phaseolina in Iran, 52 isolates of the fungus was isolated from 24 host plants across the 14 Iranian provinces. All isolates were confirmed to the species based on the species-specific primers. The aggressiveness of M. phaseolina isolates was evaluated on the common bean. Based on the pathogenicity tests, M. phaseolina isolates from  the different hosts displayed different levels of aggressiveness on the common beans. The results showed that there was significant variation in the aggressiveness of the pathogen, however there was no distinct pattern of differentiation based on the host or geographic origin linked to the virulence of the isolates, as frequently, isolates from the same host or geographic origin had different levels of aggressiveness. Inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and pathogenic patterns on common bean in the greenhouse. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the pathogenic and genotypic characteristics.

Keywords: Aggressiveness, Charcoal Rot, ISSR

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http://wdl.persiangig.com/pages/download/?dl=http://vmehdizadeh.persiangig.com/document/Intraspecies%20diversity%20of%20Macrophomina%20phaseolina%20in%20Iran.pdf

http://www.tandfonline.com/doi/abs/10.1080/03235408.2012.655146#preview