فهرستی از برخی از گونه های جنس آگاریکوس به همراه تصاویر

مقاله ۲۰۱۲: خاصیت آنتی آکیدانی عصاره قارچ های خوراکی

In Vitro Antioxidant Activities of Water-soluble Nucleotide-extract from Edible Fungi

Cui-Lin CHENG¹⁾, Zhen-Yu WANG^{1) 2)}, Li CHENG³⁾, Hai-Tian ZHAO¹⁾, Xin YANG¹⁾, Jia-Ren LIU⁴⁾, Mo-Li CUI¹⁾, Rong-Chun WANG¹⁾

1) School of Food Science and Engineering, Harbin Institute of Technology 2) School of Forestry, Northeast Forestry University 3) School of Life Science, Heilongjiang University 4) Harvard Medical School

In this study, we obtained water-soluble nucleotide-extract from six kinds of edible fungi (Agrocybe chaxingu, Lentinus edodes, Coprinus comatus gray, Agaricus bisporus, Armillariella mellea, Flammulina velutipes), and the extract from Agrocybe Chaxingu sporocarp exhibited the highest total reducing power and the most remarkable scavenging activity on 2,2′-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), which had a good dose-response relationship with the concentration of water-soluble nucleotide. When the concentration of the nucleotide was 20 mg/mL, the ABTS scavenging rate could be 90%. The nucleotide-extract was also found to exhibit remarkable scavenging activity on hydroxyl radicals (EC₅₀ = 18.5 mg/mL), superoxide anion radicals (EC₅₀ = 38.4 mg/mL) and lipid peroxidation inhibition activity (EC₅₀ = 8.1 mg/mL). Moreover, the main nucleotides in the nucleotide-extract were identified by HPLC, which were respectively AMP, CMP, GMP, UMP, ADP, GDP and GTP in a molar ratio of 1:2.17:3.49:1.7 6:0.75:4.18:1.67. These results indicated that the nucleotide would be a new antioxidant with wonderful prospects.

اولین گزارش از بیماری لکه قهوه ای قارچ دکمه ای با عامل سودوموناس ری اکتانس در ایران

هفتم اسفند ۱۳۹۱

مقاله ۲۰۰۸: استفاده از مکمل های تجاری در افزایش راندمان قارچ دکمه ای

Supplementation of first break mushroom compost with hydrolyzed protein, commercial supplements and crystalline amino acids

Abstract

Three mushroom (Agaricus bisporus) crops (Crops 1, 2, 3) were grown to evaluate the effects of re-supplementing “spent” first break compost [mushroom compost (MC)] on mushroom yield. Mushrooms were produced for one break at the Mushroom Test Demonstration Facility, the casing layer was removed and the MC was re-supplemented with hydrolyzed protein, commercial supplements and crystalline amino acids and then re-cased at the Mushroom Research Center. Sixteen supplements, including five crystalline amino acids, one amino acid blend, one egg white and four hydrolyzed proteins, Micromax^® (a micronutrient containing nine minerals) and four commercial supplements were evaluated for their effect on mushroom yield and biological efficiency. In Crop 1, mushroom yields were stimulated (49–61%) when MC was re-supplemented with 3.6% (dry wt) Pro-Fam^® H200 FG hydrolyzed soy protein, Remo’s commercial supplement, l-isoleucine (ile), egg white protein, amino blend HLA-198 and hydrolyzed whey. Significant yield reductions were observed for MC re-supplemented with 3.6% l-tyrosine, dl-methionine or l-arginine compared to the non-supplemented control. In Crop 2, mushroom yield ranged from a high of 31.3 kg/m² on MC supplemented with 3.3% Remo’s + 0.3% ile (oven dry MC) to a low of 22.6 kg/m² on non-supplemented (control) MC (38.5% difference). In Crop 3, a response surface model was used in an attempt to optimize combinations of Remo’s commercial supplement, ile and Micromax. The response surface solution for optimal yield was 2.9% Remo’s, 0.16% ile and 0.4% Micromax. Because many of the products tested performed equally well but varied substantially in their amino acid profiles, A. bisporus appears adaptable to different supplements containing both balanced and unbalanced amino acid contents, especially those rich in the branched chain amino acids. Development and improvement of supplements designed specifically for MC may allow further increases in productivity. Double cropping would ultimately lower the cost of mushroom production by reducing labor, raw materials and time required to prepare fresh Phase II compost.

اثر روش های نگهداری قارچ روی کیفیت پودر و سوپ قارچ صدفی

Effect of Pretreatments and Drying Methods on Chemical Composition and Sensory Evaluation of Oyster Mushroom (Pluerotus Oestreatus) Powder and Soup

Abstract

Practical Applications

The growing of oyster mushroom is increasing in Uganda. Mushrooms are highly perishable because of their moisture content and fragile cell structure; hence they need preservation by drying to extend the shelf life. Drying of mushrooms and production of dried mushrooms and their powders is a practical approach where farmers can add value to mushrooms and enhance their incomes.

افزایش بیان GFP در قارچ صدفی برای بررسی پروموتر لاکیز قارچی

Enhanced Green Fluorescent Protein Expression in Pleurotus ostreatus for In Vivo Analysis of Fungal Laccase Promoters

The laccase family of Pleurotus ostreatus has been widely characterized, and studies of the genes coding for laccase isoenzymes in P. ostreatus have so far led to the identification of four different genes and the corresponding cDNAs, poxc, pox1, poxa1b and poxa3. Analyses of P. ostreatus laccase promoters poxc, pox1, poxa1b and poxa3 have allowed identification of several putative response elements, and sequences of metal-responsive elements involved in the formation of complexes with fungal proteins have been identified in poxc and poxa1b promoters. In this work, development of a system for in vivo analysis of P. ostreatus laccase promoter poxc by enhanced green fluorescent protein expression is performed, based on a poly ethylene glycol-mediated procedure for fungal transformation. A quantitative measurement of fluorescence expressed in P. ostreatus transformants is hereby reported for the first time for this fungus